MiMeDB: Showing metabocard for L-2-Amino-4-methoxy-trans-3-butenoic acid (MMDBc0017579)

Record Information

Version

2.0

Status

Detected and Quantified

Creation Date

2021-05-15 07:55:23 UTC

Update Date

2025-10-07 16:06:42 UTC

Metabolite ID

MMDBc0017579

Metabolite Identification

Common Name

L-2-Amino-4-methoxy-trans-3-butenoic acid

Description

L-2-Amino-4-methoxy-trans-3-butenoic acid is a non-proteinogenic amino acid classified as a secondary metabolite. Its chemical structure features a methoxy group and a trans-butenoic acid moiety, contributing to its biological activity. This compound is synthesized by the opportunistic pathogen Pseudomonas aeruginosa, where it plays a role in various metabolic pathways, including the inhibition of growth in competing microorganisms. L-2-Amino-4-methoxy-trans-3-butenoic acid (AMB) is involved in the biosynthesis of secondary metabolites, as evidenced by gene clusters associated with its production (PMID:39597652 ). The inactivation of specific operons in Pseudomonas aeruginosa affects the expression of genes responsible for AMB synthesis, indicating its regulatory importance (PMID:37577372 ). Additionally, the condensation reaction catalyzed by non-ribosomal peptide synthetases is crucial for its biosynthesis (PMID:35354859 ). AMB has been identified as a potent antibiotic and toxin, exhibiting toxicity towards both prokaryotic and eukaryotic cells, and it induces encystment in Acanthamoeba castellanii (PMIDs:25814981, 22064067). Overall, L-2-amino-4-methoxy-trans-3-butenoic acid is a significant compound in microbial ecology and pathogenesis.

Structure

MOL SDF PDB SMILES InChI

HEADER    PROTEIN                                 15-MAY-21   NONE
TITLE     NULL                                                        
COMPND    NULL                                                        
SOURCE    NULL                                                        
KEYWDS    NULL                                                        
EXPDTA    NULL                                                        
AUTHOR    Marvin                                                      
REVDAT   1   15-MAY-21         0                                  
HETATM    1  C   UNK     0      -2.310  -1.334   0.000  0.00  0.00           C+0
HETATM    2  C   UNK     0       1.540   0.000   0.000  0.00  0.00           C+0
HETATM    3  C   UNK     0       0.000   0.000   0.000  0.00  0.00           C+0
HETATM    4  C   UNK     0       2.310   1.334   0.000  0.00  0.00           C+0
HETATM    5  C   UNK     0       3.850   1.334   0.000  0.00  0.00           C+0
HETATM    6  N   UNK     0       1.540   2.667   0.000  0.00  0.00           N+0
HETATM    7  O   UNK     0       4.620   2.667   0.000  0.00  0.00           O+0
HETATM    8  O   UNK     0       4.620   0.000   0.000  0.00  0.00           O+0
HETATM    9  O   UNK     0      -0.770  -1.334   0.000  0.00  0.00           O+0
HETATM   10  H   UNK     0       2.310  -1.334   0.000  0.00  0.00           H+0
HETATM   11  H   UNK     0      -0.770   1.334   0.000  0.00  0.00           H+0
HETATM   12  H   UNK     0       3.080  -0.000   0.000  0.00  0.00           H+0
CONECT    1    9                                                            
CONECT    2    3    4   10                                                  
CONECT    3    2    9   11                                                  
CONECT    4    2    5    6   12                                             
CONECT    5    4    7    8                                                  
CONECT    6    4                                                            
CONECT    7    5                                                            
CONECT    8    5                                                            
CONECT    9    1    3                                                       
CONECT   10    2                                                            
CONECT   11    3                                                            
CONECT   12    4                                                            
MASTER        0    0    0    0    0    0    0    0   12    0   22    0
END

Synonyms

Value	Source
2-Amino-4-methoxy-3-butenoic acid, (e)-isomer	MeSH
2-Amino-4-methoxy-3-butenoic acid	MeSH
L-2-Amino-4-methoxy-trans-3-butenoic acid	MeSH

Molecular Formula

C₅H₉NO₃

Average Mass

131.131

Monoisotopic Mass

131.058243154

IUPAC Name

(2S,3E)-2-amino-4-methoxybut-3-enoic acid

Traditional Name

(2S,3E)-2-amino-4-methoxybut-3-enoic acid

CAS Registry Number

Not Available

SMILES

[H]\C(OC)=C(\[H])[C@]([H])(N)C(O)=O

InChI Identifier

InChI=1S/C5H9NO3/c1-9-3-2-4(6)5(7)8/h2-4H,6H2,1H3,(H,7,8)/b3-2+/t4-/m0/s1

InChI Key

HLOPMQJRUIOMJO-ZPYNKGFJSA-N

Chemical Taxonomy

Description

Belongs to the class of organic compounds known as l-alpha-amino acids. These are alpha amino acids which have the L-configuration of the alpha-carbon atom.

Kingdom

Organic compounds

Super Class

Organic acids and derivatives

Class

Carboxylic acids and derivatives

Sub Class

Amino acids, peptides, and analogues

Direct Parent

L-alpha-amino acids

Alternative Parents

Substituents

L-alpha-amino acid
Unsaturated fatty acid
Fatty acid
Fatty acyl
Amino acid
Carboxylic acid
Monocarboxylic acid or derivatives
Amine
Hydrocarbon derivative
Organic oxide
Primary amine
Organooxygen compound
Organonitrogen compound
Organic oxygen compound
Primary aliphatic amine
Organic nitrogen compound
Carbonyl group
Aliphatic acyclic compound

Molecular Framework

Aliphatic acyclic compounds

External Descriptors

Not Available

Functional Ontology

Not Available

Physical Properties

State

Expected Solid

Predicted Properties

Property	Value	Source
Water Solubility	242 g/L	ALOGPS
logP	-2.5	ALOGPS
logP	-2.9	ChemAxon
logS	0.27	ALOGPS
pKa (Strongest Acidic)	2.19	ChemAxon
pKa (Strongest Basic)	8.88	ChemAxon
Physiological Charge	0	ChemAxon
Hydrogen Acceptor Count	4	ChemAxon
Hydrogen Donor Count	2	ChemAxon
Polar Surface Area	72.55 Å²	ChemAxon
Rotatable Bond Count	3	ChemAxon
Refractivity	31.64 m³·mol⁻¹	ChemAxon
Polarizability	12.52 Å³	ChemAxon
Number of Rings	0	ChemAxon
Bioavailability	Yes	ChemAxon
Rule of Five	Yes	ChemAxon
Ghose Filter	No	ChemAxon
Veber's Rule	No	ChemAxon
MDDR-like Rule	No	ChemAxon

Spectra

Not Available

Chromatographic Retention Times and Retention Indices

Retention Times

Chromatography Type	Technique	Column	Deposition Date	Retention Time (s)
Liquid Chromatography	HILIC	Waters Acquity UPLC-BEH Amide column (eluted)	2025-10-23	386.3
Liquid Chromatography	HILIC	Accucore 150 Amide HILIC (eluted)	2025-10-23	282.6
Liquid Chromatography	Reversed-phase	Hypersil Gold 1.9 µm C18 (void)	2025-10-23	63.1
Liquid Chromatography	Reversed-phase	Agilent Zorbax Eclipse Plus C18 (2.1 mm x 150 mm, 3.5 um) (eluted)	2025-10-23	259.3
Liquid Chromatography	Reversed-phase	XBridge C18 3.5u 2.1x50 mm (eluted)	2025-10-23	297.1
Liquid Chromatography	Reversed-phase	Ascentis Express C18 (void)	2025-10-23	347.3
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (void)	2025-10-23	651.8
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-23	651.7
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC (eluted)	2025-10-23	774.2
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (eluted)	2025-10-23	199.6
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-23	372.8
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (void)	2025-10-23	210.2
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (eluted)	2025-10-23	64.8
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-23	239.6
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-23	584.7
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (void)	2025-10-23	56.7
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (void)	2025-10-23	230.2
Liquid Chromatography	Reversed-phase	Kinetex Core-Shell C18 2.6 um, 3.0 x 100 mm, Phenomenex (void)	2025-10-23	780.3
Liquid Chromatography	Reversed-phase	Waters Atlantis T3 (2.1 x 150 mm, 5 um) (eluted)	2025-10-23	680.1

Retention Indices

Chromatography Type	Peak Label	SMILES	Stationary Phase	Deposition Date	Retention Index
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_1	CO/C=C/[C@H](N)C(=O)O[Si](C)(C)C	standard non polar	2025-10-23	1921.84
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_2	CO/C=C/[C@H](N[Si](C)(C)C)C(=O)O	standard non polar	2025-10-23	2080.02
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_3	CO/C=C/[C@H](N[Si](C)(C)C)C(=O)O[Si](C)(C)C	standard non polar	2025-10-23	2128.29
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_4	CO/C=C/[C@@H](C(=O)O)N([Si](C)(C)C)[Si](C)(C)C	standard non polar	2025-10-23	2272.85
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_5	CO/C=C/[C@@H](C(=O)O[Si](C)(C)C)N([Si](C)(C)C)[Si](C)(C)C	standard non polar	2025-10-23	2044.58
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_1	CO/C=C/[C@H](N)C(=O)O[Si](C)(C)C	standard polar	2025-10-23	2197.15
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_2	CO/C=C/[C@H](N[Si](C)(C)C)C(=O)O	standard polar	2025-10-23	1995.04
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_3	CO/C=C/[C@H](N[Si](C)(C)C)C(=O)O[Si](C)(C)C	standard polar	2025-10-23	1790.58
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_4	CO/C=C/[C@@H](C(=O)O)N([Si](C)(C)C)[Si](C)(C)C	standard polar	2025-10-23	2118.35
Gas Chromatography	L-2-Amino-4-methoxy-trans-3-butenoic acid_TMS_5	CO/C=C/[C@@H](C(=O)O[Si](C)(C)C)N([Si](C)(C)C)[Si](C)(C)C	standard polar	2025-10-23	2383.68

Biological Properties

Cellular Locations

Not Available

Biospecimen Locations

Not Available

Tissue Locations

Not Available

Associated OMIM IDs

114500 (Colorectal cancer)
610247 (Eosinophilic esophagitis)

Human Proteins and Enzymes

Protein ID	Protein Name	Protein Type	Uniprot ID	Species

Human Pathways

Pathways

Name	SMPDB/PathBank

Microbial Pathways

Pathways

Not Available

Metabolic Reactions

Reactions

This table shows at most 20 reactions. For the full list of associated reactions:

See All Associated Reactions

Reaction ID	Precursor	Product	Enzyme	Reaction Type	Data Source	Reference

Health Effects and Bioactivity

Health Outcome/Bioactivity	Metabolite Response/Effect	Evidence Type	Host and Biospecimen	Data Source	Reference

Microbial Sources

Superkingdom/Kingdom	Phylum	Total species	Hosts and Body Sites	Microbial Links
Bacteria/Eubacteria	Proteobacteria	1	Human feces; Human sputum; Human urine; Human supragingival plaque; Human ; Human saliva; Human mucosa	Metabolic reconstruction
Bacteria	Proteobacteria	1	Human feces; Human sputum; Human urine; Human supragingival plaque; Human ; Human saliva; Human mucosa	Metabolic reconstruction

Exposure Sources

Other Exposures

Source Type	Data Source	Reference

Host Biospecimen and Location

Host and Biospecimen	Status	Mean	Std	Min	Max	Units	Age	Sex	Health Condition	Data Source	Reference

External Links

HMDB ID

Not Available

DrugBank ID

Not Available

Phenol Explorer Compound ID

Not Available

FooDB ID

Not Available

KNApSAcK ID

C00018833

Chemspider ID

4945225

KEGG Compound ID

Not Available

BioCyc ID

Not Available

BiGG ID

Not Available

Wikipedia Link

Not Available

METLIN ID

Not Available

PubChem Compound

6440994

PDB ID

Not Available

ChEBI ID

Not Available

Food Biomarker Ontology

Not Available

CMMC Knowledgebase

Not Available

General References

van Santen JA, Poynton EF, Iskakova D, McMann E, Alsup TA, Clark TN, Fergusson CH, Fewer DP, Hughes AH, McCadden CA, Parra J, Soldatou S, Rudolf JD, Janssen EM, Duncan KR, Linington RG: The Natural Products Atlas 2.0: a database of microbially-derived natural products. Nucleic Acids Res. 2022 Jan 7;50(D1):D1317-D1323. doi: 10.1093/nar/gkab941. [PubMed:34718710 ]

Showing metabocard for L-2-Amino-4-methoxy-trans-3-butenoic acid (MMDBc0017579)

MOL for #<Metabolite:0x00005643239979d0>

3D MOL for #<Metabolite:0x00005643239979d0>

3D SDF for #<Metabolite:0x00005643239979d0>

3D-SDF for #<Metabolite:0x00005643239979d0>

PDB for #<Metabolite:0x00005643239979d0>

3D PDB for #<Metabolite:0x00005643239979d0>

SMILES for #<Metabolite:0x00005643239979d0>

INCHI for #<Metabolite:0x00005643239979d0>

Structure for #<Metabolite:0x00005643239979d0>

3D Structure for #<Metabolite:0x00005643239979d0>