MiMeDB: Showing metabocard for N-formylmaleamate (MMDBc0056148)

Record Information

Version

2.0

Status

Detected and Quantified

Creation Date

2022-06-17 20:18:04 UTC

Update Date

2025-10-07 16:09:35 UTC

Metabolite ID

MMDBc0056148

Metabolite Identification

Common Name

N-formylmaleamate

Description

N-formylmaleamate is a metabolite belonging to the class of amides. Its chemical structure features a maleamate backbone with a formyl group attached, which plays a crucial role in various biochemical pathways. Notably, N-formylmaleamate is involved in the nicotine catabolic pathway of Pseudomonas putida S16, where it is acted upon by specific enzymes. Structural, computational, and enzymatic analyses have demonstrated that N-formylmaleamate deformylase (Nfo) and maleamate amidase (Ami) are key amide hydrolases that recognize and process N-formylmaleamate and its derivatives. These enzymes facilitate the hydrolysis of the amide bond, ultimately leading to the degradation of nicotine and related compounds. The intricate interactions between N-formylmaleamate and these enzymes underscore its significance in microbial metabolism, particularly in the context of environmental bioremediation and the microbial degradation of alkaloids. (PMID:24397579 )

Structure

MOL SDF PDB SMILES InChI

HEADER    PROTEIN                                 17-JUN-22   NONE
TITLE     NULL                                                        
COMPND    NULL                                                        
SOURCE    NULL                                                        
KEYWDS    NULL                                                        
EXPDTA    NULL                                                        
AUTHOR    Marvin                                                      
REVDAT   1   17-JUN-22         0                                  
HETATM    1  C   UNK     0      -2.310  -1.334   0.000  0.00  0.00           C+0
HETATM    2  C   UNK     0      -3.080  -2.667   0.000  0.00  0.00           C+0
HETATM    3  C   UNK     0       1.540   0.000   0.000  0.00  0.00           C+0
HETATM    4  C   UNK     0      -0.770  -1.334   0.000  0.00  0.00           C+0
HETATM    5  C   UNK     0      -2.310  -4.001   0.000  0.00  0.00           C+0
HETATM    6  N   UNK     0       0.000   0.000   0.000  0.00  0.00           N+0
HETATM    7  O   UNK     0       2.310   1.334   0.000  0.00  0.00           O-1
HETATM    8  O   UNK     0       0.000  -2.667   0.000  0.00  0.00           O+0
HETATM    9  O   UNK     0      -0.770  -4.001   0.000  0.00  0.00           O+0
HETATM   10  O   UNK     0      -3.080  -5.335   0.000  0.00  0.00           O+0
HETATM   11  H   UNK     0      -3.080  -0.000   0.000  0.00  0.00           H+0
HETATM   12  H   UNK     0      -4.620  -2.667   0.000  0.00  0.00           H+0
CONECT    1    2    4   11                                                  
CONECT    2    1    5   12                                                  
CONECT    3    6    7                                                       
CONECT    4    1    6    8                                                  
CONECT    5    2    9   10                                                  
CONECT    6    3    4                                                       
CONECT    7    3                                                            
CONECT    8    4                                                            
CONECT    9    5                                                            
CONECT   10    5                                                            
CONECT   11    1                                                            
CONECT   12    2                                                            
MASTER        0    0    0    0    0    0    0    0   12    0   22    0
END

Synonyms

Value	Source
(Z)-4-Formamido-4-oxobut-2-enoate	ChEBI
N-Formylmaleamate anion	ChEBI
(Z)-4-Formamido-4-oxobut-2-enoic acid	Generator
N-Formylmaleamic acid anion	Generator
N-Formylmaleamic acid	Generator

Molecular Formula

C₅H₄NO₄

Average Mass

142.091

Monoisotopic Mass

142.014581193

IUPAC Name

N-[(2Z)-3-carboxyprop-2-enoyl]methanecarboximidate

Traditional Name

N-[(2Z)-3-carboxyprop-2-enoyl]methanecarboximidate

CAS Registry Number

Not Available

SMILES

[H]\C(=C(/[H])C(=O)N=C[O-])C(O)=O

InChI Identifier

InChI=1S/C5H5NO4/c7-3-6-4(8)1-2-5(9)10/h1-3H,(H,9,10)(H,6,7,8)/p-1/b2-1-

InChI Key

HSKSAKBZUITULZ-UPHRSURJSA-M

Chemical Taxonomy

Description

Belongs to the class of organic compounds known as n-acyl amines. N-acyl amines are compounds containing a fatty acid moiety linked to an amine group through an ester linkage.

Kingdom

Organic compounds

Super Class

Lipids and lipid-like molecules

Class

Fatty Acyls

Sub Class

Fatty amides

Direct Parent

N-acyl amines

Alternative Parents

Substituents

Fatty acid
N-acyl-amine
Unsaturated fatty acid
Straight chain fatty acid
Carboxylic acid imide
Dicarboximide
Carboxylic acid imide, n-unsubstituted
Carboxylic acid derivative
Carboxylic acid
Monocarboxylic acid or derivatives
Carbonyl group
Organic nitrogen compound
Organonitrogen compound
Organooxygen compound
Hydrocarbon derivative
Organic oxide
Organopnictogen compound
Organic oxygen compound
Organic anion
Aliphatic acyclic compound

Molecular Framework

Aliphatic acyclic compounds

External Descriptors

monocarboxylic acid anion (CHEBI:59911 )

Functional Ontology

Not Available

Physical Properties

State

Expected Solid

Predicted Properties

Property	Value	Source
logP	-0.54	ChemAxon
pKa (Strongest Acidic)	2.77	ChemAxon
pKa (Strongest Basic)	-2.3	ChemAxon
Physiological Charge	-1	ChemAxon
Hydrogen Acceptor Count	5	ChemAxon
Hydrogen Donor Count	1	ChemAxon
Polar Surface Area	89.79 Å²	ChemAxon
Rotatable Bond Count	2	ChemAxon
Refractivity	42.38 m³·mol⁻¹	ChemAxon
Polarizability	11.64 Å³	ChemAxon
Number of Rings	0	ChemAxon
Bioavailability	Yes	ChemAxon
Rule of Five	Yes	ChemAxon
Ghose Filter	No	ChemAxon
Veber's Rule	No	ChemAxon
MDDR-like Rule	No	ChemAxon

Spectra

Spectrum Type	Description	Deposition Date	View
1D NMR	¹³C NMR Spectrum (1D, 100 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 100 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 1000 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 1000 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 200 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 200 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 300 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 300 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 400 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 400 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 500 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 500 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 600 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 600 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 700 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 700 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 800 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 800 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 900 MHz, H₂O, predicted)	2022-08-18	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 900 MHz, H₂O, predicted)	2022-08-18	View Spectrum

Chromatographic Retention Times and Retention Indices

Retention Times

Not Available

Retention Indices

Not Available

Biological Properties

Cellular Locations

Not Available

Biospecimen Locations

Not Available

Tissue Locations

Not Available

Associated OMIM IDs

114500 (Colorectal cancer)
610247 (Eosinophilic esophagitis)

Human Proteins and Enzymes

Protein ID	Protein Name	Protein Type	Uniprot ID	Species

Human Pathways

Pathways

Name	SMPDB/PathBank

Microbial Pathways

Pathways

Not Available

Metabolic Reactions

Reactions

This table shows at most 20 reactions. For the full list of associated reactions:

See All Associated Reactions

Reaction ID	Precursor	Product	Enzyme	Reaction Type	Data Source	Reference

Health Effects and Bioactivity

Health Outcome/Bioactivity	Metabolite Response/Effect	Evidence Type	Host and Biospecimen	Data Source	Reference

Microbial Sources

Superkingdom/Kingdom	Phylum	Total species	Hosts and Body Sites	Microbial Links
Bacteria/Eubacteria	Proteobacteria	1

Exposure Sources

Other Exposures

Source Type	Data Source	Reference

Host Biospecimen and Location

Host and Biospecimen	Status	Mean	Std	Min	Max	Units	Age	Sex	Health Condition	Data Source	Reference

External Links

HMDB ID

Not Available

DrugBank ID

Not Available

Phenol Explorer Compound ID

Not Available

FooDB ID

Not Available

KNApSAcK ID

Not Available

Chemspider ID

25069055

KEGG Compound ID

Not Available

BioCyc ID

Not Available

BiGG ID

Not Available

Wikipedia Link

Not Available

METLIN ID

Not Available

PubChem Compound

46173390

PDB ID

Not Available

ChEBI ID

59911

Food Biomarker Ontology

Not Available

CMMC Knowledgebase

Not Available

General References

Bansal P, Morgat A, Axelsen KB, Muthukrishnan V, Coudert E, Aimo L, Hyka-Nouspikel N, Gasteiger E, Kerhornou A, Neto TB, Pozzato M, Blatter MC, Ignatchenko A, Redaschi N, Bridge A: Rhea, the reaction knowledgebase in 2022. Nucleic Acids Res. 2022 Jan 7;50(D1):D693-D700. doi: 10.1093/nar/gkab1016. [PubMed:34755880 ]

Showing metabocard for N-formylmaleamate (MMDBc0056148)

MOL for #<Metabolite:0x00007fadc8013c18>

3D MOL for #<Metabolite:0x00007fadc8013c18>

3D SDF for #<Metabolite:0x00007fadc8013c18>

3D-SDF for #<Metabolite:0x00007fadc8013c18>

PDB for #<Metabolite:0x00007fadc8013c18>

3D PDB for #<Metabolite:0x00007fadc8013c18>

SMILES for #<Metabolite:0x00007fadc8013c18>

INCHI for #<Metabolite:0x00007fadc8013c18>

Structure for #<Metabolite:0x00007fadc8013c18>

3D Structure for #<Metabolite:0x00007fadc8013c18>