MiMeDB: Showing metabocard for 2-Keto-glutaramic acid (MMDBc0032001)

Microbial

Animal

Record Information

Version

2.0

Status

Detected and Quantified

Creation Date

2021-11-18 01:58:11 UTC

Update Date

2025-11-05 17:13:02 UTC

Metabolite ID

MMDBc0032001

Metabolite Identification

Common Name

2-Keto-glutaramic acid

Description

2-Keto-glutaramic acid is a dicarboxylic acid and a key metabolite in the amino acid metabolism pathway. Its chemical structure features a keto group adjacent to a carboxylic acid, which is crucial for its biochemical reactivity. This compound plays a role in various metabolic processes, particularly in the metabolism of glutamate, arginine, and aspartate. Notably, studies have shown that 2-keto-glutaramic acid levels are significantly elevated in inflammatory osteoarthritis (OA) patients, indicating a potential disruption in glutamine metabolism associated with OA progression (PMID:33655418 ). Additionally, gut microbiota such as Bacteroides vulgatus and Campylobacter jejuni can influence glutamate metabolism, leading to decreased levels of 2-keto-glutaramic acid (PMID:32290475 ). Furthermore, alterations in glutamate metabolites, including a decline in 2-keto-glutaramic acid, have been observed in individuals with autism spectrum disorder (ASD), suggesting a link between microbiota composition and glutamate metabolism (PMID:30701194 ). Lastly, oxidative stress has been shown to deplete 2-keto-glutaramic acid levels in brain tissues, highlighting its relevance in neurological contexts (PMID:30607820 ).

Structure

MOL 3D MOL SDF 3D SDF PDB 3D PDB SMILES InChI

Synonyms

Value	Source
2-Ketoglutaramate	ChEBI
alpha-Ketoglutaramate	ChEBI
2-Ketoglutaramic acid	Generator
2-keto-Glutaramate	Generator, HMDB
a-Ketoglutaramate	Generator
a-Ketoglutaramic acid	Generator
alpha-Ketoglutaramic acid	Generator
α-Ketoglutaramate	Generator
α-Ketoglutaramic acid	Generator
2-Oxoglutaramate	HMDB, Generator
2-Oxoglutaramic acid	KEGG
4-Oxoglutaramic acid	HMDB
5-Amino-2,5-dioxopentanoic acid	HMDB

Molecular Formula

C₅H₇NO₄

Average Mass

145.1134

Monoisotopic Mass

145.037507717

IUPAC Name

4-carbamoyl-2-oxobutanoic acid

Traditional Name

2-keto-glutaramate

CAS Registry Number

Not Available

SMILES

NC(=O)CCC(=O)C(O)=O

InChI Identifier

InChI=1S/C5H7NO4/c6-4(8)2-1-3(7)5(9)10/h1-2H2,(H2,6,8)(H,9,10)

InChI Key

COJBGNAUUSNXHX-UHFFFAOYSA-N

Chemical Taxonomy

Description

Belongs to the class of organic compounds known as short-chain keto acids and derivatives. These are keto acids with an alkyl chain the contains less than 6 carbon atoms.

Kingdom

Organic compounds

Super Class

Organic acids and derivatives

Class

Keto acids and derivatives

Sub Class

Short-chain keto acids and derivatives

Direct Parent

Short-chain keto acids and derivatives

Alternative Parents

Substituents

Short-chain keto acid
Alpha-keto acid
Fatty acyl
Fatty amide
Alpha-hydroxy ketone
Carboxamide group
Primary carboxylic acid amide
Ketone
Carboxylic acid derivative
Carboxylic acid
Monocarboxylic acid or derivatives
Organic nitrogen compound
Organonitrogen compound
Organooxygen compound
Hydrocarbon derivative
Organic oxide
Organopnictogen compound
Carbonyl group
Organic oxygen compound
Aliphatic acyclic compound

Molecular Framework

Aliphatic acyclic compounds

External Descriptors

2-oxo monocarboxylic acid (CHEBI:30882 )

Functional Ontology

Not Available

Physical Properties

State

Expected Solid

Predicted Properties

Property	Value	Source
Water Solubility	26.7 g/L	ALOGPS
logP	-1.4	ALOGPS
logP	-0.92	ChemAxon
logS	-0.74	ALOGPS
pKa (Strongest Acidic)	2.97	ChemAxon
pKa (Strongest Basic)	-1.9	ChemAxon
Physiological Charge	-1	ChemAxon
Hydrogen Acceptor Count	4	ChemAxon
Hydrogen Donor Count	2	ChemAxon
Polar Surface Area	97.46 Å²	ChemAxon
Rotatable Bond Count	4	ChemAxon
Refractivity	30.71 m³·mol⁻¹	ChemAxon
Polarizability	12.56 Å³	ChemAxon
Number of Rings	0	ChemAxon
Bioavailability	Yes	ChemAxon
Rule of Five	Yes	ChemAxon
Ghose Filter	No	ChemAxon
Veber's Rule	No	ChemAxon
MDDR-like Rule	No	ChemAxon

Spectra

Spectrum Type	Description	Splash Key	Deposition Date	View
Predicted GC-MS	Predicted GC-MS Spectrum - GC-MS (Non-derivatized) - 70eV, Positive	splash10-0f6x-9300000000-364f00f83f2890864bf3	2017-09-01	View Spectrum
Predicted GC-MS	Predicted GC-MS Spectrum - GC-MS (1 TMS) - 70eV, Positive	splash10-0uk9-9600000000-f15721df49885ecdf65e	2017-10-06	View Spectrum
Predicted GC-MS	Predicted GC-MS Spectrum - GC-MS (Non-derivatized) - 70eV, Positive	Not Available	2021-10-12	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 10V, Positive	splash10-004i-1900000000-2eb098e06d8301b0bce6	2015-09-14	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 20V, Positive	splash10-06w9-6900000000-d802d6bf63187e0b9714	2015-09-14	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 40V, Positive	splash10-0ab9-9000000000-0aaaa6f3f4d3183b6b21	2015-09-14	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 10V, Negative	splash10-0006-2900000000-c4ab5bdf984680f19900	2015-09-15	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 20V, Negative	splash10-0f96-9800000000-6a2a8c2f9da457c041c2	2015-09-15	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 40V, Negative	splash10-0006-9000000000-76ca31bd526f5a4086c8	2015-09-15	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 10V, Negative	splash10-0udl-5900000000-bfedace6bca6e0663444	2021-09-24	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 20V, Negative	splash10-0006-9100000000-0a497925699a66249231	2021-09-24	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 40V, Negative	splash10-0006-9000000000-90726b17dc36e29c5299	2021-09-24	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 10V, Positive	splash10-0fbc-6900000000-a420c6f7f5db4cb58165	2021-09-24	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 20V, Positive	splash10-0ac3-9100000000-e9bdcc615e9a7032177c	2021-09-24	View Spectrum
Predicted LC-MS/MS	Predicted LC-MS/MS Spectrum - 40V, Positive	splash10-0k97-9000000000-02445ea4ef20f8ea72fb	2021-09-24	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 100 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 100 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 1000 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 1000 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 200 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 200 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 300 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 300 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 400 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 400 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 500 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 500 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 600 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 600 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 700 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 700 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 800 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 800 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹³C NMR Spectrum (1D, 900 MHz, D₂O, predicted)		2021-09-29	View Spectrum
1D NMR	¹H NMR Spectrum (1D, 900 MHz, D₂O, predicted)		2021-09-29	View Spectrum

Chromatographic Retention Times and Retention Indices

Retention Times

Chromatography Type	Technique	Column	Deposition Date	Retention Time (s)
Liquid Chromatography	HILIC	Waters Acquity UPLC-BEH Amide column (eluted)	2025-10-23	400.0
Liquid Chromatography	HILIC	Accucore 150 Amide HILIC (eluted)	2025-10-23	226.3
Liquid Chromatography	Reversed-phase	Hypersil Gold 1.9 µm C18 (void)	2025-10-23	52.3
Liquid Chromatography	Reversed-phase	Agilent Zorbax Eclipse Plus C18 (2.1 mm x 150 mm, 3.5 um) (eluted)	2025-10-23	256.9
Liquid Chromatography	Reversed-phase	XBridge C18 3.5u 2.1x50 mm (eluted)	2025-10-23	244.5
Liquid Chromatography	Reversed-phase	Ascentis Express C18 (void)	2025-10-23	304.9
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (void)	2025-10-23	738.0
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-23	698.9
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC (eluted)	2025-10-23	713.6
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (eluted)	2025-10-23	166.5
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-23	393.4
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (void)	2025-10-23	185.3
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (eluted)	2025-10-23	68.4
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-23	304.6
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-23	609.3
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (void)	2025-10-23	59.3
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (void)	2025-10-23	189.0
Liquid Chromatography	Reversed-phase	Kinetex Core-Shell C18 2.6 um, 3.0 x 100 mm, Phenomenex (void)	2025-10-23	747.0
Liquid Chromatography	Reversed-phase	Waters Atlantis T3 (2.1 x 150 mm, 5 um) (eluted)	2025-10-23	579.0

Retention Indices

Chromatography Type	Peak Label	SMILES	Stationary Phase	Deposition Date	Retention Index
Gas Chromatography	2-Keto-glutaramic acid_TMS_1	C[Si](C)(C)OC(=O)C(=O)CCC(N)=O	standard non polar	2025-10-24	1955.14
Gas Chromatography	2-Keto-glutaramic acid_TMS_2	C[Si](C)(C)OC(=CCC(N)=O)C(=O)O	standard non polar	2025-10-24	1884.78
Gas Chromatography	2-Keto-glutaramic acid_TMS_3	C[Si](C)(C)NC(=O)CCC(=O)C(=O)O	standard non polar	2025-10-24	2037.4
Gas Chromatography	2-Keto-glutaramic acid_TMS_4	C[Si](C)(C)OC(=O)C(=CCC(N)=O)O[Si](C)(C)C	standard non polar	2025-10-24	2121.55
Gas Chromatography	2-Keto-glutaramic acid_TMS_5	C[Si](C)(C)NC(=O)CCC(=O)C(=O)O[Si](C)(C)C	standard non polar	2025-10-24	1772.71
Gas Chromatography	2-Keto-glutaramic acid_TMS_6	C[Si](C)(C)NC(=O)CC=C(O[Si](C)(C)C)C(=O)O	standard non polar	2025-10-24	1894.94
Gas Chromatography	2-Keto-glutaramic acid_TMS_7	C[Si](C)(C)N(C(=O)CCC(=O)C(=O)O)[Si](C)(C)C	standard non polar	2025-10-24	2214.74
Gas Chromatography	2-Keto-glutaramic acid_TMS_8	C[Si](C)(C)NC(=O)CC=C(O[Si](C)(C)C)C(=O)O[Si](C)(C)C	standard non polar	2025-10-24	1675.95
Gas Chromatography	2-Keto-glutaramic acid_TMS_9	C[Si](C)(C)OC(=O)C(=O)CCC(=O)N([Si](C)(C)C)[Si](C)(C)C	standard non polar	2025-10-24	2440.6
Gas Chromatography	2-Keto-glutaramic acid_TMS_10	C[Si](C)(C)OC(=CCC(=O)N([Si](C)(C)C)[Si](C)(C)C)C(=O)O	standard non polar	2025-10-24	2072.98
Gas Chromatography	2-Keto-glutaramic acid_TMS_11	C[Si](C)(C)OC(=O)C(=CCC(=O)N([Si](C)(C)C)[Si](C)(C)C)O[Si](C)(C)C	standard non polar	2025-10-24	2183.06
Gas Chromatography	2-Keto-glutaramic acid_TMS_1	C[Si](C)(C)OC(=O)C(=O)CCC(N)=O	standard polar	2025-10-24	1888.9
Gas Chromatography	2-Keto-glutaramic acid_TMS_2	C[Si](C)(C)OC(=CCC(N)=O)C(=O)O	standard polar	2025-10-24	2075.44
Gas Chromatography	2-Keto-glutaramic acid_TMS_3	C[Si](C)(C)NC(=O)CCC(=O)C(=O)O	standard polar	2025-10-24	1490.54
Gas Chromatography	2-Keto-glutaramic acid_TMS_4	C[Si](C)(C)OC(=O)C(=CCC(N)=O)O[Si](C)(C)C	standard polar	2025-10-24	2015.19
Gas Chromatography	2-Keto-glutaramic acid_TMS_5	C[Si](C)(C)NC(=O)CCC(=O)C(=O)O[Si](C)(C)C	standard polar	2025-10-24	2018.11
Gas Chromatography	2-Keto-glutaramic acid_TMS_6	C[Si](C)(C)NC(=O)CC=C(O[Si](C)(C)C)C(=O)O	standard polar	2025-10-24	2483.53
Gas Chromatography	2-Keto-glutaramic acid_TMS_7	C[Si](C)(C)N(C(=O)CCC(=O)C(=O)O)[Si](C)(C)C	standard polar	2025-10-24	1803.81
Gas Chromatography	2-Keto-glutaramic acid_TMS_8	C[Si](C)(C)NC(=O)CC=C(O[Si](C)(C)C)C(=O)O[Si](C)(C)C	standard polar	2025-10-24	2070.67
Gas Chromatography	2-Keto-glutaramic acid_TMS_9	C[Si](C)(C)OC(=O)C(=O)CCC(=O)N([Si](C)(C)C)[Si](C)(C)C	standard polar	2025-10-24	2257.72
Gas Chromatography	2-Keto-glutaramic acid_TMS_10	C[Si](C)(C)OC(=CCC(=O)N([Si](C)(C)C)[Si](C)(C)C)C(=O)O	standard polar	2025-10-24	1703.56
Gas Chromatography	2-Keto-glutaramic acid_TMS_11	C[Si](C)(C)OC(=O)C(=CCC(=O)N([Si](C)(C)C)[Si](C)(C)C)O[Si](C)(C)C	standard polar	2025-10-24	1858.38

Biological Properties

Cellular Locations

Extracellular
Membrane

Biospecimen Locations

Feces

Tissue Locations

Not Available

Associated OMIM IDs

114500 (Colorectal cancer)
610247 (Eosinophilic esophagitis)

Human Proteins and Enzymes

Protein ID	Protein Name	Protein Type	Uniprot ID	Species
MMDBp0192123	Kynurenine--oxoglutarate transaminase 1	Enzyme	Q16773	Homo sapiens
MMDBp0196912	Omega-amidase NIT2	Unknown	Q9NQR4

Human Pathways

Pathways

Name	SMPDB/PathBank

Microbial Pathways

Pathways

Name	SMPDB/PathBank	Species
L-Glutamate Metabolism		Escherichia coli Escherichia coli SMS-3-5 Escherichia coli UTI89 Escherichia coli 55989 Escherichia coli SE11 Escherichia coli CFT073 Shigella boydii Escherichia albertii Escherichia coli E24377A Escherichia coli O127:H6 str. E2348/69 Escherichia coli UMN026 Escherichia coli ED1a Escherichia coli IAI39 Escherichia coli 042 Shigella sonnei Ss046 Shigella flexneri 5 str. 8401 Escherichia albertii TW07627 Shigella flexneri 2a str. 301 Shigella boydii Sb227

Metabolic Reactions

Reactions

This table shows at most 20 reactions. For the full list of associated reactions:

See All Associated Reactions

Reaction ID	Precursor	Product	Enzyme	Reaction Type	Data Source	Reference
MMDBr0026144	2-Keto-glutaramic acid	L-Glutamine	Ornithine aminotransferase	Amine group addition	RHEA	34755880
MMDBr0036142	L-Glutamine	2-Keto-glutaramic acid	L-amino acid dehydrogenase	Dehydrogenation	RHEA	34755880

Health Effects and Bioactivity

Health Outcome/Bioactivity	Metabolite Response/Effect	Evidence Type	Host and Biospecimen	Data Source	Reference

Microbial Sources

Superkingdom/Kingdom	Phylum	Total species	Hosts and Body Sites	Microbial Links
Bacteria/Eubacteria	Proteobacteria	7	Human ; Human feces; Human feces; pleural fluid plaque; bal	Unknown; Metabolic reconstruction
Bacteria/Eubacteria	Bacteroidetes	71	Human feces; Human appendix biopsy; Human ; Human gastric fluid; Human saliva; Human subgingival plaque; Human supragingival plaque	Metabolic reconstruction
Bacteria/Eubacteria	Firmicutes	24	Human feces; Human
Bacteria	Actinobacteria	6
Bacteria/Eubacteria	Actinobacteria	5
Eukaryota/Fungi	Ascomycota	4

Exposure Sources

Other Exposures

Source Type	Data Source	Reference

Host Biospecimen and Location

Host and Biospecimen	Status	Mean	Std	Min	Max	Units	Age	Sex	Health Condition	Data Source	Reference
Human Feces	Detected but not Quantified									HMDB

External Links

HMDB ID

HMDB0001552

DrugBank ID

Not Available

Phenol Explorer Compound ID

Not Available

FooDB ID

FDB022686

KNApSAcK ID

Not Available

Chemspider ID

KEGG Compound ID

C00940

BioCyc ID

2-KETO-GLUTARAMATE

BiGG ID

Not Available

Wikipedia Link

Not Available

METLIN ID

6318

PubChem Compound

PDB ID

Not Available

ChEBI ID

30882

Food Biomarker Ontology

Not Available

CMMC Knowledgebase

Not Available

General References

Noronha A, Modamio J, Jarosz Y, Guerard E, Sompairac N, Preciat G, Danielsdottir AD, Krecke M, Merten D, Haraldsdottir HS, Heinken A, Heirendt L, Magnusdottir S, Ravcheev DA, Sahoo S, Gawron P, Friscioni L, Garcia B, Prendergast M, Puente A, Rodrigues M, Roy A, Rouquaya M, Wiltgen L, Zagare A, John E, Krueger M, Kuperstein I, Zinovyev A, Schneider R, Fleming RMT, Thiele I: The Virtual Metabolic Human database: integrating human and gut microbiome metabolism with nutrition and disease. Nucleic Acids Res. 2019 Jan 8;47(D1):D614-D624. doi: 10.1093/nar/gky992. [PubMed:30371894 ]

Showing metabocard for 2-Keto-glutaramic acid (MMDBc0032001)

MOL for #<Metabolite:0x00007f9ac0afd1d0>

3D MOL for #<Metabolite:0x00007f9ac0afd1d0>

3D SDF for #<Metabolite:0x00007f9ac0afd1d0>

3D-SDF for #<Metabolite:0x00007f9ac0afd1d0>

PDB for #<Metabolite:0x00007f9ac0afd1d0>

3D PDB for #<Metabolite:0x00007f9ac0afd1d0>

SMILES for #<Metabolite:0x00007f9ac0afd1d0>

INCHI for #<Metabolite:0x00007f9ac0afd1d0>

Structure for #<Metabolite:0x00007f9ac0afd1d0>

3D Structure for #<Metabolite:0x00007f9ac0afd1d0>