MiMeDB: Showing metabocard for 2-Methyl-3-hydroxybutanoic acid (MMDBc0060387)

Microbial

Record Information

Version

2.0

Status

Detected and Quantified

Creation Date

2023-02-14 16:56:17 UTC

Update Date

2025-10-07 20:47:39 UTC

Metabolite ID

MMDBc0060387

Metabolite Identification

Common Name

2-Methyl-3-hydroxybutanoic acid

Description

2-Methyl-3-hydroxybutanoic acid is a carboxylic acid that serves as a key metabolite in various biochemical pathways. Its chemical structure features a branched carbon chain with a hydroxyl group and a carboxylic acid functional group, which contributes to its reactivity and biological roles. This compound is involved in the acylation of tetrasaccharides, as demonstrated by its incorporation into the structure of Tyrianthinic acid VI, where it is bonded to a jalapinolic acid (PMID:24838512 ). Such modifications are significant in the biosynthesis of complex carbohydrates, influencing their properties and functions in biological systems. The presence of 2-methyl-3-hydroxybutanoic acid in metabolic pathways underscores its relevance in the synthesis of diverse biomolecules, contributing to cellular processes and signaling mechanisms.

Structure

MOL SDF PDB SMILES InChI

Synonyms

Value	Source
(2S)-Methyl-(3S)-hydroxybutanoate	ChEBI
(2S,3S)-3-Hydroxy-2-methylbutyrate	ChEBI
(2S)-Methyl-(3S)-hydroxybutanoic acid	Generator
(2S,3S)-3-Hydroxy-2-methylbutyric acid	Generator
(2S,3S)-3-Hydroxy-2-methylbutanoic acid	Generator

Molecular Formula

C₅H₉O₃

Average Mass

117.125

Monoisotopic Mass

117.05571773

IUPAC Name

Not Available

Traditional Name

Not Available

CAS Registry Number

Not Available

SMILES

Not Available

InChI Identifier

Not Available

InChI Key

VEXDRERIMPLZLU-IMJSIDKUSA-M

Chemical Taxonomy

Description

Belongs to the class of organic compounds known as hydroxy fatty acids. These are fatty acids in which the chain bears a hydroxyl group.

Kingdom

Organic compounds

Super Class

Lipids and lipid-like molecules

Class

Fatty Acyls

Sub Class

Fatty acids and conjugates

Direct Parent

Hydroxy fatty acids

Alternative Parents

Substituents

Beta-hydroxy acid
Branched fatty acid
Hydroxy fatty acid
Methyl-branched fatty acid
Short-chain hydroxy acid
Hydroxy acid
Secondary alcohol
Carboxylic acid derivative
Carboxylic acid
Monocarboxylic acid or derivatives
Hydrocarbon derivative
Alcohol
Carbonyl group
Organic oxide
Organic oxygen compound
Organooxygen compound
Organic anion
Aliphatic acyclic compound

Molecular Framework

Aliphatic acyclic compounds

External Descriptors

2-methyl-3-hydroxybutyrate (CHEBI:78559 )

Functional Ontology

Not Available

Physical Properties

State

Not Available

Predicted Properties

Not Available

Spectra

Not Available

Chromatographic Retention Times and Retention Indices

Retention Times

Chromatography Type	Technique	Column	Deposition Date	Retention Time (s)
Liquid Chromatography	HILIC	Waters Acquity UPLC-BEH Amide column (eluted)	2025-10-24	199.1
Liquid Chromatography	HILIC	Accucore 150 Amide HILIC (eluted)	2025-10-24	66.2
Liquid Chromatography	Reversed-phase	Hypersil Gold 1.9 µm C18 (void)	2025-10-24	287.8
Liquid Chromatography	Reversed-phase	Agilent Zorbax Eclipse Plus C18 (2.1 mm x 150 mm, 3.5 um) (eluted)	2025-10-24	362.0
Liquid Chromatography	Reversed-phase	XBridge C18 3.5u 2.1x50 mm (eluted)	2025-10-24	276.8
Liquid Chromatography	Reversed-phase	Ascentis Express C18 (void)	2025-10-24	344.3
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (void)	2025-10-24	1180.6
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-24	108.0
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC (eluted)	2025-10-24	668.2
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (eluted)	2025-10-24	259.9
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-24	274.4
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (eluted)	2025-10-24	202.1
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (eluted)	2025-10-24	85.0
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-24	196.0
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-24	472.3
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (void)	2025-10-24	54.7
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (void)	2025-10-24	264.3
Liquid Chromatography	Reversed-phase	Kinetex Core-Shell C18 2.6 um, 3.0 x 100 mm, Phenomenex (eluted)	2025-10-24	918.3
Liquid Chromatography	Reversed-phase	Waters Atlantis T3 (2.1 x 150 mm, 5 um) (eluted)	2025-10-24	678.6

Retention Indices

Not Available

Biological Properties

Cellular Locations

Not Available

Biospecimen Locations

Not Available

Tissue Locations

Not Available

Associated OMIM IDs

114500 (Colorectal cancer)
610247 (Eosinophilic esophagitis)

Human Proteins and Enzymes

Protein ID	Protein Name	Protein Type	Uniprot ID	Species

Human Pathways

Pathways

Name	SMPDB/PathBank

Microbial Pathways

Pathways

Not Available

Metabolic Reactions

Reactions

This table shows at most 20 reactions. For the full list of associated reactions:

See All Associated Reactions

Reaction ID	Precursor	Product	Enzyme	Reaction Type	Data Source	Reference
MMDBr0038827	2-Methyl-3-hydroxybutyryl-CoA	2-Methyl-3-hydroxybutanoic acid	Thioesterase TesA	Hydrolysis	RHEA	34755880

Health Effects and Bioactivity

Health Outcome/Bioactivity	Metabolite Response/Effect	Evidence Type	Host and Biospecimen	Data Source	Reference

Microbial Sources

Superkingdom/Kingdom	Phylum	Total species	Hosts and Body Sites	Microbial Links

Exposure Sources

Other Exposures

Source Type	Data Source	Reference

Host Biospecimen and Location

Host and Biospecimen	Status	Mean	Std	Min	Max	Units	Age	Sex	Health Condition	Data Source	Reference

External Links

HMDB ID

Not Available

DrugBank ID

Not Available

Phenol Explorer Compound ID

Not Available

FooDB ID

Not Available

KNApSAcK ID

Not Available

Chemspider ID

Not Available

KEGG Compound ID

Not Available

BioCyc ID

Not Available

BiGG ID

Not Available

Wikipedia Link

Not Available

METLIN ID

Not Available

PubChem Compound

86289592

PDB ID

Not Available

ChEBI ID

78559

Food Biomarker Ontology

Not Available

CMMC Knowledgebase

Not Available

General References

Showing metabocard for 2-Methyl-3-hydroxybutanoic acid (MMDBc0060387)

MOL for #<Metabolite:0x00007f2e514c4010>

3D MOL for #<Metabolite:0x00007f2e514c4010>

3D SDF for #<Metabolite:0x00007f2e514c4010>

3D-SDF for #<Metabolite:0x00007f2e514c4010>

PDB for #<Metabolite:0x00007f2e514c4010>

3D PDB for #<Metabolite:0x00007f2e514c4010>

SMILES for #<Metabolite:0x00007f2e514c4010>

INCHI for #<Metabolite:0x00007f2e514c4010>

Structure for #<Metabolite:0x00007f2e514c4010>

3D Structure for #<Metabolite:0x00007f2e514c4010>